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Biology

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  • Rat Atrium: Courtesy of Jan Redick, Central EM Facility, UVA. Rat atrium fixed in 2% paraformaldehyde + 0.5% glutaraldehyde and embedded in Lowicryl K4M. Primary antibody was rabbit anti-atrial natrurietic peptide, followed by Protein A conjugated to 10 nm colloidal gold. Camera is side-mounted.

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  • Paneth cells: Courtesy of Jan Redick, Central EM Facility, University of Virginia. Two adjacent Paneth cells at the base of a crypt in the duodenum. The large granules in the cells are secretory granules and contain the lysozyme. Image taken on a JEOL 100C TEM. Camera is side-mounted.

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  • Courtesy of Christine Davis, Center for Advanced Microscopy, University of Virginia. Portions of three tumor cells. The center cell contains four unknown bacteria. In the upper right hand corner is a portion of a small blood vessel. The lumen of the vessel is the blank space and the dark nucleus is the nucleus of the endothelial cell lining the vessel. Camera is side-mounted.
  • Fixed in 4%PF + 2.5% Glut
  • Post fix in osmium tetroxide.
  • Embedded in EPON
  • Counterstained with UA and Pb

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  • Replica of fibropectin protein sprayed onto mica chips; TEM magnification: 100kx
  • Image courtesy of Martin Schwartz and Christine Davis, Advanced Microscopy Facility, UVA

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Material Science

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